TIM-1 Antibody | 3809

TIM-1 Antibody | 3809 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (56.3%)

Immunogen: Anti-TIM-1 antibody (3809) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1.
The immunogen is located within amino acids 50 - 100 of TIM-1.

Research Area: Immunology, Neuroscience, Signal Transduction, Growth Factors

Tested Application: E, WB, IHC-P, IF

Application: WB: 1 - 8 μg/mL (overnight incubation at 4˚ C) ; IHC-P: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 1203 - A549 Cell Lysate

Positive Control 3: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4: Cat. No. 11-401 - Human Uterus Tissue Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 44kD
Observed: 60kD (Post-modification: 4 N-linked glycosylation)

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. 

siRNA knockdown validation (Figure 3) : Anti-TIM-1 antibody (3809) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs.

Isoform: Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD) , isoform a (364aa, 39kD) , isoform b precursor (401aa, 44kD) , isoform b (359aa, 39kD) , and isoform c (359aa, 39kD) . This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD) , isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .

Purification: TIM-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TIM-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1) , a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.