TIM-3 Antibody | 46-495

TIM-3 Antibody | 46-495 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog

Immunogen: The immunogen for this antibody is: C-KWYSHSKEKIQN

Research Area: Cancer

Tested Application: E, WB, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell lines Jurkat and MOLT-4 and in Human Tonsil lysates (calculated MW of 33.4kDa according to NP_116171.3) . Recommended concentration:1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 (squamous carcinoma) cell lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3

User Note: N/A

BACKGROUND: N/A