TIM-4, human recombinant | P1108

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. TIM-4 (T cell; immunoglobulin; Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells.

P1108 | TIM-4 human recombinant DataSheet

Biomolecule/Target: TIMD4

Synonyms: TIM4; TIMD4, T Cell Immunoglobulin and Mucin Domain-containing Protein 4, T Cell Membrane Protein 4

Alternates names: PWTSR, THSD2, Roof plate-specific spondin-3, RSPO3

Taglines: Involved in regulating T-cell proliferation and lymphotoxin signaling.

NCBI Gene ID #: 84870

NCBI Gene Symbol: RSPO3

Gene Source: Human

Accession #: Q9BXY4

Recombinant: Yes

Source: CHO Cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

Biological activity: R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 37.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH