TIMELESS (Human) ELISA Kit - E5016

E5016 | TIMELESS (Human) ELISA Kit DataSheet

TIMELESS (TIM) is a core component of the circadian clock. It is necessary for generating circadian rhythms and photo-entrainment in flies. In flies, TIM and PER proteins form a heterodimer and contribute to the negative feedback loop of the circadian clock. In humans, TIM is required for the electrical oscillations output by the suprachiasmatic nucleus (SCN). Human TIM also plays a critical role in DNA replication, stabilizes replication forks by forming a complex with TIPIN, and promotes DNA repair. In mice, TIM is necessary for embryonic development. Homozygous knockout of TIM in mice is embryonically lethal, whereas heterozygous TIM knockout does not affect the circadian period. Studies have demonstrated that TIM is frequently overexpressed in cancers such as breast, colon, lung, and cervical cancer. Decreasing TIMELESS expression slows the proliferation of cancer cells. Hence, TIMELESS may serve as an efficient marker for anti-cancer therapies. ’s TIMELESS (Human) ELISA kit quantitatively measures TIMELESS in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of TIMELESS in the sample or standard.

Alternate Name : TIM, TIM1, hTIM, TIMELESS, TIMELESS1

Tag Line : A Sandwich ELISA kit for quantitative measurement of TIMELESS in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma Serum Other biological fluids Tissue lysate

Species Reactivity : Human

Applications : Sandwich ELISA kit for quantitative measurement of TIMELESS in serum, plasma, cell and tissue lysates, and other biological fluids

Features and Benefits : Detection range: 0.156 – 10 ng/ml Recovery range: 85 - 105% for normal human serum and plasma samples Sensitivity: 0.094 ng/ml Assay Precision: Intra-Assay CV and Inter-Assay CV < 10% This Sandwich ELISA is highly sensitive and highly specific for the detection of TIMELESS in human samples. There is no significant cross-reactivity or interference between TIMELESS and analogues