TMB ELISA Substrate | 42-TB08

TMB ELISA Substrate | 42-TB08 |

Activity Code: ACTIVE

Product Type: Biological Reagents

Product Subtype: Substrates

Research Area: Cardiac Markers

Short Description: High sensitivity HRP microwell substrate (containing TMB)

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: Supplied as a liquid.

Storage: Store at 4 deg C.

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end user.  This product should be allowed to equilibrate to room temperature (25°C) prior to use. For microwell applications, 100 uL of substrate solution is added to each well. A soluble blue reaction product develops which can be read at 370 nm or in a range of 620 nm to 650 nm. For best results, sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units. In endpoint assays, the substrate reaction can be stopped using equal volumes of 1 N HCl, 0.6 N sulfuric acid. Addition of acid turns the blue color to yellow and stops the enzymatic reaction. Since stopping the reaction increases sample absorbance values approximately three fold sample OD values should be monitored and substrate reaction stopped when values reach approximately 0.7 OD units. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.

Assay Information: N/A