TMB, High Kinetics | 1216

3,3',5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe, sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mol⁻¹ cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹ ) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBHK, available from Inc., is a highly sensitive single component reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. TMBHK is stable at room temperature for six months and is not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP commonly employed in immunologic assays.

TMB, High Kinetics has been shown to be a safe, sensitive substrate for the assay of horseradish peroxidase (HRP).

1216 | TMB, High Kinetics DataSheet

Alternate Name/Synonyms: 3,3',5,5'-Tetramethylbenzidine; TMBHK

Appearance: Liquid

Formulation: 2.5 mMol L⁻¹ TMB and Hydrogen Peroxide in a proprietary buffer at pH 3.6.

CAS Number: 54827-17-7

Structure Available?: No

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₆H₂₀N₂

Molecular Weight: 240.35

Cell-Permeable?: No

Purity: N/A

Solubilities: N/A

Handling: Protect from light

Country of Origin: USA

Tag Line: A peroxidase substrate

MDL Number: MFCD00007748

PubChem CID: 41206

SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

InChi: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChi Key: UAIUNKRWKOVEESUHFFFAOYSA-N