Description
TMP21 Antibody | 46-502 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-YLRRFFKAKKLIE
Research Area: Cancer
Tested Application: E, IF, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: TMED10, transmembrane emp24-like trafficking protein 10 (yeast) , P24 (DELTA) , S31I125, S31III125, TMP21, Tmp-21-I, 21 kDa transmembrane trafficking protein, transmembrane emp24 domain-containing protein 10
User Note: N/A
BACKGROUND: N/A