TNF-a Antibody | XP-5284

TNF-a Antibody | XP-5284 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mTNF-alpha (murine TNF-alpha) .

Research Area: Immunology, Chemokines & Cytokines, Signal Transduction, Growth Factors, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
To detect mTNF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mTNF-alpha.
Sandwich:
To detect mTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine TNF-α (XP-5284Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-α.
Western Blot:
To detect mTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-mTNF alpha specific antibody was purified by affinity chromatography employing immobilized mTNF-alpha matrix.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: TNF-a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tnfsf2, Tumor necrosis factor, Cachectin

User Note: Centrifuge vial prior to opening.

BACKGROUND: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.