TNF-alpha Extracellular Domain, Human Recombinant | P1395

Tumor Necrosis Factor- (TNF-) is a highly pleiotropic cytokine with multiple activities other than its originally discovered role of tumor necrosis in rodents. It contributes to both inflammations during infections and anti-inflammatory/tissue repair processes after clearance of infection. TNF- is mainly produced by activated macrophages, T lymphocytes, and natural killer (NK) cells. The activity of TNF- is mediated through two cell surface receptors, TNF-R1 (p55) and TNF-R2 (p75) that differ in their signaling activity. TNF-'s primary role is in the regulation of immune cells. It can induce cell death of certain tumor cell lines. About 28% of cancers are susceptible to direct cell killing mediated by soluble TNF-.

P1395 | TNF-alpha Extracellular Domain Human Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

Alternates names: soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)

Taglines: A potent cytokine exerting cytotoxic effects on a wide range of cells

NCBI Gene ID #: 8600

NCBI Gene Symbol: RANKL

Gene Source: Human

Accession #: O14788

Recombinant: False

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit sRANKL induced NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect in the presence of 15 ng/ml of recombinant sRANKL, is 30-50 ng/ml.

Biological activity: Determined by its ability to inhibit sRANKL induced NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect in the presence of 15 ng/ml of recombinant sRANKL, is 30-50 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.2.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK