TNFa | Tumor necrosis factor alpha, Biotin conjugated | IMS06-102-325

TNFa | Tumor necrosis factor alpha, Biotin conjugated | IMS06-102-325 | Gentaur UK, US & Europe Distribution

Immunogen: purified full length recombinant protein P01375

Host: Chicken

Conjugation: Biotin

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide., pH 7.2.

Format: Liquid

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: IMS01-102-339 | Anti-TNFa | Tumor necrosis factor alpha, chicken antibodiesIMS01-142-310 | Anti-Etanercept (Enbrel®), recombinant human tumor necrosis factor receptor, chicken antibodies

Recommended Dilutions: 1 : 5000 (WB)

Molecular weight: 26 kDa

Confirmed Reactivity: Human

Predicted Reactivity: Cat, Dog, Horse, Pig, Primates

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: The antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots

Background: Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Reconstitution: N/A

Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Cancer

Research Area: Cancer Research