TNNI3 Antibody | 43-244

TNNI3 Antibody | 43-244 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat, Pig

Homology: Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen: The immunogen for this antibody is: C-KVTKNITEIAD

Research Area: Signal Transduction, Stem Cell

Tested Application: E, WB, IF

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25+26kDa doublet band observed in Human Heart, approx 24+27kDa in Mouse Heart, approx 25kDa in Rat Heart and approx 27kDa in Pig Heart lysates (calculated MW of 24kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3kDa according to Mouse NP_033432.1 and Ra tNP_058840.1 ) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 27 + 28kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TNNI3, troponin I type 3 (cardiac) , CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle

User Note: N/A

BACKGROUND: N/A