Toc159 | Chloroplast outer envelope membrane translocon complex protein | AS07 239

Toc159 | Chloroplast outer envelope membrane translocon complex protein | AS07 239 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)

Host: Guinea pig

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

Related Products: AS06 150 | Anti-Toc75 (monocots and dicots) antibodiesAS08 292 | Anti-Toc75 (Pisum sativum) antibodies

Recommended Dilutions: 1 : 500 (CM), 1 : 10 000 (WB)

Molecular weight: 150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx, 29 kDa degradation product (cross reaction with GTPase domain)

Confirmed Reactivity: Pisum sativum

Predicted Reactivity: Dicots, Oryza sativa Species of your interest not listed? Contact us

Not reactive in: Arabidopsis thaliana

Additional Information: Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance.

Background: Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Chaperones/Transporters

Research Area: Plant Biology, Photosynthesis