Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 | AS08 345

Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 | AS08 345 | Gentaur UK, US & Europe Distribution

Immunogen: psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Flow cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)

Related Products: AS07 236 | Anti-Toc33 | chloroplast outer envelope membrane translocon complex protein, chicken antibodiesAS07 235 | Anti-Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain), chicken antibodiesAS08 292 | Anti-Toc75 | Outer chloroplast membrane translocon complex protein, rabbit antibodiesAS08 351 | Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)

Molecular weight: 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Confirmed Reactivity: Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus

Predicted Reactivity: Ricinus communis, Vitis vinifera, Populus trichocarpa Species of your interest not listed? Contact us

Not reactive in: Physcomitrella patens

Additional Information: Antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction

Background: OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446) ; In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303) ; FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Chaperones/Transporters

Research Area: Plant Biology, Photosynthesis