LBD
TOP1 Antibody (N-term) [APR03883G]
- SKU:
- LBD-APR03883G
- Availability:
- Usually ships in 5 working days
Description
TOP1 Antibody (N-term) [APR03883G] | Gentaur UK, US & Europe Distribution
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1.
Cellular Localisation: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli
Molecular Weight: 90726
Clone: Polyclonal
Gene Name: TOP1
Gene ID: 7150
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) - enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) . Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Summary: Tissue Location: Endothelial cells..
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IF
Dilution: WB--1:1000 IF--1:10~50
Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1