TOP2 | DNA topoisomerase II | AS05 096

TOP2 | DNA topoisomerase II | AS05 096 | Gentaur UK, US & Europe Distribution

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

Related Products: AS07 225 | Anti-A12.2 | RNA polymerase I subunit (homolog of Pol II Rpb9), rabbit antibodiesAS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodiesPlant protein extraction buffer

Recommended Dilutions: 1 : 500 (IL), 1 : 2000 (WB)

Molecular weight: 164 | 170 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Vicia faba

Predicted Reactivity: Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrium patens, Pinus sitcHensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox Caterii Species of your interest not listed? Contact us

Not reactive in: Nicotiana tabacum

Additional Information: Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.This product can be sold containing ProClin if requested.

Background: Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G23890

Category: Transcription regulation

Research Area: Plant Biology