Description
TOPAZ1 Antibody | 7737 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (88%) , Sheep: (88%)
Immunogen: TOPAZ1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TOPAZ1.
The immunogen is located within amino acids 1330 - 1380 of TOPAZ1.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: TOPAZ1 antibody can be used for detection of TOPAZ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificiy: TOPAZ1 antibody is human, mouse and rat reactive. At least three isoforms of TOPAZ1 are known to exist.
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 65, 186 kDa
Observed: 60, 240 kDa
Validation: N/A
Isoform: N/A
Purification: TOPAZ1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TOPAZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TOPAZ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: TOPAZ1 Antibody: C3orf77, C3orf77, Testis- and ovary-specific PAZ domain-containing protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The testis and ovary specific PAZ domain containing 1 (TOPAZ1) protein is a highly conserved protein that is highly expressed in the gonads during germ cell meiosis, suggesting that it may play a role in germ cell development (1) . It contains a PAZ domain and a zinc finger domain and is exclusively expressed in adult testis and fetal ovary.