Topo IIIβ-1 (Q84) polyclonal Antibody | BS1908

Topo IIIβ-1 (Q84) polyclonal Antibody | BS1908 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III α exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III α, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III β exists as three isoforms, namely β1, β2 and β3, also produced by alternative splicing. DNA topoisomerase III β1 is expressed in testes, heart and skeletal muscle, whereas β2 is expressed in thymus, kidney and pancreas.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Topo IIIβ-1 (Q84) polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein.

Molecular Weight: ~ 96 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; TOP3B; TOP3B1

Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human Topo IIIβ-1.

Conjugate: N/A

Modification: N/A

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A