Toso Antibody | 2273

Toso Antibody | 2273 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Toso antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Toso.
The immunogen is located within the first 50 amino acids of Toso.

Research Area: Apoptosis

Tested Application: E, WB

Application: Toso antibody can be used for detection of Toso by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 43 kDa
Observed: 41 kDa

Validation: N/A

Isoform: N/A

Purification: Toso Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Toso Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Toso antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Toso Antibody: FCMR, TOSO, Fas apoptotic inhibitory molecule 3, Regulator of Fas-induced apoptosis Toso

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Toso Antibody: Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD) - containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.