TRAF2 Antibody | 46-510

TRAF2 Antibody | 46-510 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-KMEAKNSYVRDD

Research Area: Cancer

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9kDa; according to Human NP_066961.2) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75µg/ml. Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1316 - Human Ovary Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TRAF2, TNF receptor-associated factor 2, MGC:45012, TRAP, TRAP3, OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3

User Note: N/A

BACKGROUND: N/A