Description
TRAF2 Antibody | 46-510 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-KMEAKNSYVRDD
Research Area: Cancer
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9kDa; according to Human NP_066961.2) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75µg/ml. Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1316 - Human Ovary Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: TRAF2, TNF receptor-associated factor 2, MGC:45012, TRAP, TRAP3, OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3
User Note: N/A
BACKGROUND: N/A