TraKine™ F-actin Staining Kit (Green Fluorescence) | KTC4008

TraKine™ F-actin Staining Kit (Green Fluorescence) | KTC4008 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Staining

Product Type: Cell Staining/Tracing Kit

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Kit Component: • Phalloidin (Orange Fluorescence) (200×)
• Assay Buffer (5×)

Features & Benefits : • Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
• Proprietary Phalloidin (Orange Fluorescence) (Ex/Em = 593/614nm) -high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.

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Usage Note: As the optimal staining conditions may vary among different cell types, we recommend that a suitable concentration of Phalloidin (Orange Fluorescence) be determined.

Storage Conditions: Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

Shipping: Gel pack with blue ice.

Background: Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides) . It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.

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Tag: F-actin