TraKine™ F-actin Staining Kit (Orange Fluorescence) | KTC4009

TraKine™ F-actin Staining Kit (Orange Fluorescence) | KTC4009 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Staining

Product Type: Cell Staining/Tracing Kit

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Kit Component: • TubGreen™ (200 uM)
• Buffer T

Features & Benefits : • Optimized staining protocol for labeling Tubulin in mammalian living cells. The product has been tested in U2OS, Hela, COS-7 and ARPE cell lines. U2OS cell line is preferred. If the sample type is not included in the above cell lines, we can provide experimental services for specific cell lines.
• Suitable for Confocal high-definition imaging.
• Suitable for structured light micro imaging (SIM) live cell research, ultra-high resolution microscopic imaging of live cells, and dynamic observation of cells in three-dimensional space.
• Proprietary TubGreen™ (Ex/Em = 500/520 nm) -high specificity, low background and excellent photostability.
• Low levels of cytotoxicity.

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Usage Note: Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.

Storage Conditions: Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

Shipping: Gel pack with blue ice.

Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

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Tag: Tubulin