Description
Tribo™ Tryptase Activity Assay | TBS2101
Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.
Applications:
- Cell culture supernatants, cell lysates, or other tryptase containing supplies.
- Testing of purified tryptase enzyme, in vitro inhibitor screening.
- Cell degranulation
Key Features:
- Sensitive and Accurate: The kit detects as low as 10μM in solution.
- Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.
Kit Contents:
(for 100x assays)
Storage Conditions:
Store the kit at -20℃ protected from life
Shelf life: 6 months.
Example of pNA Standard Curve
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Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.
Applications:
- Cell culture supernatants, cell lysates, or other tryptase containing supplies.
- Testing of purified tryptase enzyme, in vitro inhibitor screening.
- Cell degranulation
Key Features:
- Sensitive and Accurate: The kit detects as low as 10μM in solution.
- Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.
Kit Contents:
(for 100x assays)
| Component | Amount |
|---|---|
| 5x Assay Buffer | 20 mL |
| Tryptase inhibitor | 200μL (10mM) |
| Tryptase Substrate | 2mL (2.5mM) |
| pNA Standard | 100μL (10mM) |
| Calcium Ionophore | 50μL (1mM) |
| Tryptase Positive Control | 200μL (10 μg/mL) |
Storage Conditions:
Store the kit at -20℃ protected from life
Shelf life: 6 months.
Example of pNA Standard Curve
Example Tryptase Positive Control Curve
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