TRIM2 Antibody | 46-519

TRIM2 Antibody | 46-519 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Rat

Homology: Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow

Immunogen: The immunogen for this antibody is: DSGNHCFKVYRYLQ

Research Area: Cancer

Tested Application: E, WB, IHC, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75-85kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1) . An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Specificiy: Cross-reactivity with TRIM3 has been demonstrated in Mouse.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2

User Note: N/A

BACKGROUND: N/A