TRIM28 Antibody | 2521

TRIM28 Antibody | 2521 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Rabbit polyclonal TRIM28 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TRIM28.
The immunogen is located within the last 50 amino acids of TRIM28.

Research Area: Stem Cell

Tested Application: E, WB

Application: TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of TRIM28 are known to exist; this antibody will detect all three isoforms

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 92 kDa

Validation: N/A

Isoform: N/A

Purification: TRIM28 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TRIM28 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TRIM28 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRIM28 Antibody: KAP1, TF1B, RNF96, TIF1B, KAP1, Transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, TIF1-beta

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB) -domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR) , retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.