TRIM29 Antibody | 42-505

TRIM29 Antibody | 42-505 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow

Immunogen: The immunogen for this antibody is: C-EKDRIKSFTTNE

Research Area: Cancer

Tested Application: E, WB, IF, Flow, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1) . Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room tempetature.Immunohistochemistry:In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29

User Note: N/A

BACKGROUND: N/A