TRIM33 Antibody | 7385

TRIM33 Antibody | 7385 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33.
The immunogen is located within amino acids 580 - 630 of TRIM33.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 10-201 - Human Liver Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 70, 124 kDa
Observed: 70 kDa

Validation: N/A

Isoform: N/A

Purification: TRIM33 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TRIM33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TRIM33 Antibody: TRIM24 (TIF1alpha) , TRIM28 (TIF1beta) , and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2) . The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3) . TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3) . It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4) . It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5) .