TRIP12 Antibody | 7953

TRIP12 Antibody | 7953 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.
The immunogen is located within amino acids 1930 - 1980 of TRIP12.

Research Area: Homeostasis, Stem Cell

Tested Application: E, WB, IHC-P, IF, ICC

Application: TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

Positive Control 1: Cat. No. 1472 - Rat Colon Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 219 kDa
Observed: 220 kDa

Validation: N/A

Isoform: N/A

Purification: TRIP12 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TRIP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRIP12 Antibody: ULF, TRIP-12, KIAA0045, ULF, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1) . TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2, 3) . TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4) . TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5) .