Trophinin Antibody | 8245

Trophinin Antibody | 8245 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Trophinin antibody was raised against a 16 amino acid peptide near the amino terminus of human Trophinin.
The immunogen is located within amino acids 390 - 440 of Trophinin.

Research Area: Stem Cell, Cancer, Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: Trophinin antibody can be used for detection of Trophinin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Trophinin antibody is human, mouse and rat reactive. Multiple isoforms of Trophinin are known to exist.

Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 78, 157 kDa
Observed: 94 kDa

Validation: N/A

Isoform: N/A

Purification: Trophinin antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Trophinin antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Trophinin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRO, MAGE-d3, MAGED3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Trophinin, also known as TRO or MAGED3, is an apical cell adhesion molecule that is implicated in the initial attachment during the process of embryo implantation and functions by mediating cell adhesion between trophoblastic and endometrial epithelial cells (1, 2) . Trophinin is a membrane protein expressed in chorionic villi trophoblasts and in maternal endometrial epithelial cells in an implantation-dependent manner and interacts with Bystin and Tastin, facilitating cell adhesion and embryo implantation (3, 4) . The induction of Trophinin expression may be a useful method for improving implantation rates and can be a potential diagnostic factor and biomarker for human cancer (4, 5) .