TRPC3 Antibody | 8269

TRPC3 Antibody | 8269 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
The immunogen is located within amino acids 710 - 760 of TRPC3.

Research Area: Signal Transduction, Neuroscience

Tested Application: E, WB, IHC-P

Application: TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.

Positive Control 1: Cat. No. 1362 - Human Cerebellum Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 90, 93, 101 kDa
Observed: 93 kDa

Validation: N/A

Isoform: N/A

Purification: TRPC3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TRPC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.