TRPV4 Antibody | 7695

TRPV4 Antibody | 7695 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%) , Mouse: (100%)

Immunogen: TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4.
The immunogen is located within amino acids 380 - 430 of TRPV4.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 96 kDa
Observed: 100 kDa

Validation: N/A

Isoform: N/A

Purification: TRPV4 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TRPV4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TRPV4 Antibody: SMAL, VRL2, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Osm-9-like TRP channel 4, TrpV4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical) , V type (vanilloid receptor related) , M type (melastatin related) and P type (PKD) (1) . TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2, 3) . TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3) . TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4) .