TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic) | AS14 2809

TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic) | AS14 2809 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana TrxM1 UniProt: O48737, TAIR: AT1G03680 and TrxM2 UniProt:Q9SEU8, TAIR: AT4G03520

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS14 2808 | Anti-Trxf1/2 | Thioredoxin F1/F2, chloroplastic, rabbit antibodiesAS06 159 | Anti-NtrC | thioredoxin reductase (TR/TRxR), rabbit antibodiesCollection of antibodies to chloroplastic proteinsPlant and algal protein extraction buffer

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 20 | 14 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica napus, Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Populus balsamifera, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Theobroma cacao, Zea mays, Viola bifloraSpecies of your interest not listed? Contact us

Not reactive in: Marchantia polymorpha, Physcomitrella patens

Additional Information: 5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.To work with this antibody chloroplast fraction has to be used.

Background: TrxM1/M2 (Thioredoxin M1/M2, chloroplasticT) is a thiol-disulfide oxidoreductase involved in the redox regulation of enzymes of both reductive pentose phosphate pathway (Calvin-Benson cycle) and oxidative pentose phosphate pathway. Under reducing conditions, activates the glyceraldehyde-3-phosphate dehydrogenase and the phosphoribulokinase, and inhibits. the glucose-6-phosphate dehydrogenase. Activates NADP-malate dehydrogenase.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G03680

Category: RUBISCO/Carbon metabolism

Research Area: Plant Biology