Trypsin, Recombinant, Proteomics grade | P1228

Trypsin hydrolyzes peptides, amides, and ester bonds involving the carboxyl groups of L-arginine or L-lysine. Increased serum values of this enzyme and/or its zymogen (trypsinogen) have been found in patients with cystic fibrosis. Trypsinogen has three isoforms: cationic (trypsinogen 1), anionic (trypsinogen 2, MW - 22.5 kDa), and mesotrypsinogen (trypsinogen 3, MW - 25 kDa).

P1228 | Trypsin Recombinant Proteomics grade DataSheet

Biomolecule/Target: Trypsin

Synonyms: Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I, PRSS1, TRP1, TRY1, TRYP1

Alternates names: SEMA3A, SEMAD

Taglines: Hydrolyzes peptides, amides, and ester bonds involving the carboxyl groups of L-arginine or L-lysine

NCBI Gene ID #: 10371

NCBI Gene Symbol: SEMA3A

Gene Source: Human

Accession #: Q14563

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay.

Biological activity: Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~90 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: NYQNGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF DLVNIKDFQK IVWPVSYTRR DECKWAGKDI LKECANFIKV LKAYNQTHLY ACGTGAFHPI CTYIEIGHHP EDNIFKLENS HFENGRGKSP YDPKLLTASL LIDGelYSGT AADFMGRDFA IFRTLGHHHP IRTEQHDSRW LNDPKFISAH LISESDNPED DKVYFFFREN AIDGEHSGKA THARIGQICK NDFGGHRSLV NKWTTFLKAR LICSVPGPNG IDTHFDELQD VFLMNFKDPK NPVVYGVFTT SSNIFKGSAV CMYSMSDVRR VFLGPYAHRD GPNYQWVPYQ GRVPYPRPGT CPSKTFGGFD STKDLPDDVI TFARSHPAMY NPVFPMNNRP IVIKTDVNYQ FTQIVVDRVD AEDGQYDVMF IGTDVGTVLK VVSIPKETWY DLEEVLLEEM TVFREPTAIS AMELSTKQQQ LYIGSTAGVA QLPLHRCDIY GKACAECCLA RDPYCAWDGS ACSRYFPTAK RRTRRQDIRN GDPLTHCSDL HHDNHHGHSP EERIIYGVEN SSTFLECSPK SQRALVYWQF QRRNEERKEE IRVDDHIIRT DQGLLLRSLQ QKDSGNYLCH AVEHGFIQTL LKVTLEVIDT EHLEELLHKD DDGDGSKTKE MSNSMTPSQK VWYRDFMQLI NHPNLNTMDE FCEQVWKRDR KQRRQRPGHT PGNSNKWKHL QENKKGRNRR THEFERAPRS V