Tryptophan Hydroxylase (phospho Ser19) Antibody | 50-242

Tryptophan Hydroxylase (phospho Ser19) Antibody | 50-242 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse, Rat, Bovine, Frog

Homology: N/A

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2) .

Research Area: Phospho-Specific, Neuroscience

Tested Application: WB

Application: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 55

Validation: N/A

Isoform: N/A

Purification: Affinity Purified

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: N/A

Concentration: N/A

Storage Condition: Tryptophan Hydroxylase antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001) . In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) . CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et