TSC2 Antibody | 3507

TSC2 Antibody | 3507 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (86%)

Immunogen: TSC2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2.
The immunogen is located within amino acids 1450 - 1500 of TSC2.

Research Area: Signal Transduction

Tested Application: E, WB

Application: TSC2 antibody can be used for the detection of TSC2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1284 - L1210 Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 200 kDa
Observed: 200 kDa

Validation: N/A

Isoform: N/A

Purification: TSC2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TSC2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TSC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TSC2 Antibody: LAM, TSC4, Tuberin, Tuberous sclerosis 2 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR) , an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.