TSLP Antibody | 4025

TSLP Antibody | 4025 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse, Rat

Homology: N/A

Immunogen: Anti-TSLP antibody (4025) was raised against a peptide corresponding to 20 amino acids near the center of mouse TSLP.
The immunogen is located within the amino acids 70-120 of TSLP.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5 - 2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 16 kD
Observed: 24 kD (Post-modifications: 3 N-linked glycosylations)

Validation: N/A

Isoform: Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD) . 4025 can detect mouse and rat isoform.

Purification: TSLP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TSLP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TSLP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TSLP Antibody: Thymic stromal lymphopoietin

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.