TTBK1 Antibody | 5013

TTBK1 Antibody | 5013 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: TTBK1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TTBK1.
The immunogen is located within amino acids 1190 - 1240 of TTBK1.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 145, 151 kDa
Observed: 140 kDa

Validation: N/A

Isoform: N/A

Purification: TTBK1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TTBK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TTBK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TTBK1 Antibody: BDTK, BDTK, KIAA1855, Tau-tubulin kinase 1, Brain-derived tau kinase

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TTBK1 Antibody: Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients.