TUNEL Apoptosis Detection Kit (Green Fluorescence) | KTA2010

TUNEL Apoptosis Detection Kit (Green Fluorescence) | KTA2010 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cellular states

Product Type: Apoptosis Detection Kit

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Kit Component: • TdT Enzyme
• Equilibration Buffer (5X)
• Label Mix Orange

Features & Benefits : • Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.
• Signal can be easily detected (Ex/Em = 555nm/565 nm) .

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Usage Note: The reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using.

Storage Conditions: Stable for at least 6 months at -20°C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

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Tag: TUNEL