UbcH3, human recombinant (His-tag) | 4849

UbcH3 is a class II enzyme, homologous to Cdc34 from Saccharomyces cerevisiae, and is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. UbcH3 substrates that have been characterized include IB, Wee1, ICERII, p27Xic1. Additionally, substrates such as ß-catenin, p21, and is phosphorylated and ubiquitinylated in vivo.

4849 | UbcH3 human recombinant His-tag DataSheet

Biomolecule/Target: N/A

Synonyms: UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme

Alternates names: UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, CDC34, UBCH3, UBE2R1

Taglines: An essential ubiquitin-conjugating enzyme

NCBI Gene ID #: 8743

NCBI Gene Symbol: TNF10

Gene Source: Human

Accession #: P50591

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The ED for the effect is 1-3 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.8 kDa

Concentration: 0.6 mg/ml

Appearance: Liquid

Physical form description: Supplied at a concentration of 0.5 mg/ml in 50 mM HEPES, 50 mM NaCl, pH 8.0, containing 1.0 mM DTT.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A