UbcH5a, human recombinant (His-tag) (New size) | 4851

UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.

4851 | UbcH5a human recombinant His-tag DataSheet

Biomolecule/Target: N/A

Synonyms: UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme

Alternates names: UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1

Taglines: An essential ubiquitin-conjugating enzyme

NCBI Gene ID #: 8742

NCBI Gene Symbol: TNFSF12

Gene Source: Human

Accession #: O43508

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.0 kDa

Concentration: 1.0 mg/ml

Appearance: Liquid

Physical form description: Supplied as a liquid in 20 mM Tris-HCl, pH 8, containing 0.5 mM DTT.

Reconstitution Instructions: Reconstitute in 10 mM Sodium Phosphate (pH 7.5) to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A