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UbcH5a, human recombinant (His-tag) (New size) | 4851
- SKU:
- 26-4851-GEN
- Availability:
- Usually shipped in 5 working days
Description
UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
4851 | UbcH5a human recombinant His-tag DataSheet
Biomolecule/Target: N/A
Synonyms: UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme
Alternates names: UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1
Taglines: An essential ubiquitin-conjugating enzyme
NCBI Gene ID #: 8742
NCBI Gene Symbol: TNFSF12
Gene Source: Human
Accession #: O43508
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17.0 kDa
Concentration: 1.0 mg/ml
Appearance: Liquid
Physical form description: Supplied as a liquid in 20 mM Tris-HCl, pH 8, containing 0.5 mM DTT.
Reconstitution Instructions: Reconstitute in 10 mM Sodium Phosphate (pH 7.5) to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A