UbcH5c, human recombinant (His-tag) | 4854

UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100 nM to 1 µM depending upon the conditions.

4854 | UbcH5c human recombinant His-tag DataSheet

Biomolecule/Target: N/A

Synonyms: UbcH5c, Ubiquitin conjugating enzyme H5c, Ubiquitin conjugating enzyme

Alternates names: UbcH5c, Ubiquitin conjugating enzyme H5c, Ubiquitin conjugating enzyme, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3

Taglines: An essential ubiquitin-conjugating enzyme

NCBI Gene ID #: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: Q96KJ0

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.2 kDa

Concentration: 1.0 mg/ml

Appearance: Liquid

Physical form description: Supplied at a concentration of 1.0 mg/ml in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A