UBD Antibody | 8185

UBD Antibody | 8185 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (81%)

Immunogen: UBD antibody was raised against a 16 amino acid peptide near the center of human UBD.
The immunogen is located within amino acids 80 - 130 of UBD.

Research Area: Apoptosis, Immunology

Tested Application: E, WB, IHC-P, IF

Application: UBD antibody can be used for detection of UBD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: UBD antibody is human specific.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 18 kDa
Observed: 17 kDa

Validation: N/A

Isoform: N/A

Purification: UBD antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: UBD antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: UBD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Ubiquitin D, Diubiquitin, FAT10, GABBR1, UBD-3, Ubiquitin-like protein FAT10

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: UBD, also designated ubiquitin D or diubiquitin, plays a role in antigen presentation, cytokine response and apoptosis. It is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin (1, 2) . UBD is inducible by interferon gamma and TNF-alpha and regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (3) . It may also regulate cell growth during dendritic cell or B cell activation and development (4, 5) .