Ubiquitin-AMC | 4842

Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039M) and for Isopeptidase-T (Km = 0.17-1.4M), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.

4842 | Ubiquitin-AMC DataSheet

Biomolecule/Target: N/A

Synonyms: Ubiquitin-Aminomethylcoumarin

Alternates names: Ubiquitin-Aminomethylcoumarin

Taglines: An industry standard substrate for deubiquitinylating enzymes (DUBs)

NCBI Gene ID #: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: Q96KJ0

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: N/A

Purity: 95%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.2 kDa

Concentration: 1 mg/ml (DMSO)

Appearance: Liquid

Physical form description: A solution in DMSO ( 1 mg/ml)

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A