Ubiquitin-Rhodamine | 6411

Ubiquitin-rhodamine 110 is a quenched, fluorescent substrate for deubiquitylases, especially ubiquitin C-terminal hydrolases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm). Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

6411 | Ubiquitin-Rhodamine DataSheet

Biomolecule/Target: N/A

Synonyms: Ubiquitin-Rhodamine

Alternates names: N/A

Taglines: Industry standard substrate for deubiquitinylating enzymes

NCBI Gene ID #: 22339

NCBI Gene Symbol: VEGFA

Gene Source: N/A

Accession #: Q00731

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: RP-HPLC

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the mitogenic activity on human mbilical vein endothelial cells and bovine aortic endothelial cells. Mitogenic activity was obtained for stimulation of 3H-thymidine incorporation and cell proliferation in a concentration range of 1.0 to 5.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 39.0 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A