Ubiquitin-TAMRA-labeled (TMR-Ub) | 7553

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, ’s TAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

7553 | Ubiquitin-TAMRA-labeled TMR-Ub DataSheet

Biomolecule/Target: Ubiquitin

Synonyms: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.

Alternates names: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.

Taglines: Substrate to determine the activity of ubiquitin conjugating enzymes

NCBI Gene ID #: 83785

NCBI Gene Symbol: VEGFA

Gene Source: N/A

Accession #: P16612

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: RP-HPLC

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 10-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.4 kDa

Concentration: 8 mg/mL

Appearance: Liquid

Physical form description: 8 mg/mL in PBS

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A