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Ubiquitin-TAMRA-labeled (TMR-Ub) | 7553
- SKU:
- 26-7553-GEN
- Availability:
- Usually shipped in 5 working days
Description
Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, s TAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.
7553 | Ubiquitin-TAMRA-labeled TMR-Ub DataSheet
Biomolecule/Target: Ubiquitin
Synonyms: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.
Alternates names: UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.
Taglines: Substrate to determine the activity of ubiquitin conjugating enzymes
NCBI Gene ID #: 83785
NCBI Gene Symbol: VEGFA
Gene Source: N/A
Accession #: P16612
Recombinant: N/A
Source: N/A
Purity by SDS-PAGEs: 98%
Assay: RP-HPLC
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 10-100 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 38.4 kDa
Concentration: 8 mg/mL
Appearance: Liquid
Physical form description: 8 mg/mL in PBS
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A