UBQ11 | Ubiquitin (serum) | AS08 307

UBQ11 | Ubiquitin (serum) | AS08 307 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS10 307A | antt-UBQ11 | Ubiquitin, affinity purified, rabbit antibodyAS08 308 | Anti-SUMO1 | Small ubiquitin-like modifier protein 1, rabbit antibodiesAS08 349 | Anti-SUMO3 | Small ubiquitin-like modifier protein 3, rabbit antibodies

Recommended Dilutions: 1 : 10 000 (WB)

Molecular weight: 8, 5 kDa

Confirmed Reactivity: Arabidopsis thaliana, Euphorbia kansui, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum

Predicted Reactivity: Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Olea europaea var. sylvestris, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: Algae

Additional Information: Suggested extraction buffer: 100mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50mM sodium metabisulfite, 20mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018) . This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.

Background: Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS) .

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G05050

Category: Autophagy-related and Ubiquitin-like Proteins

Research Area: Plant Biology