UCP1 Antibody | 7697

UCP1 Antibody | 7697 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1 (GenBank accession.
The immunogen is located within the last 50 amino acids of UCP1.

Research Area: Obesity

Tested Application: E, WB, IHC-P

Application: UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: UCP1 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-201 - Human Liver Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 34 kDa
Observed: 35 kDa

Validation: N/A

Isoform: N/A

Purification: UCP1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: UCP1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: UCP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: UCP1 Antibody: UCP, SLC25A7, UCP, Mitochondrial brown fat uncoupling protein 1, Solute carrier family 25 member 7, UCP 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1) . UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1) . UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1) .