UQCRFS1 Antibody (C-term) [APR10672G]

UQCRFS1 Antibody (C-term) [APR10672G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This UQCRFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-209 amino acids from the C-terminal region of human UQCRFS1.

Cellular Localisation: Mitochondrion inner membrane; Single-pass membrane protein {ECO:0000250|UniProtKB:Q5ZLR5}

Molecular Weight: 29668

Clone: Polyclonal

Gene Name: UQCRFS1 (HGNC:12587)

Gene ID: 7386

Function: [Cytochrome b-c1 complex subunit Rieske, mitochondrial]: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed:31883641) . The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b- c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron- sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q (0) site in cytochrome b to cytochrome c1 (By similarity) . Incorporation of UQCRFS1 is the penultimate step in complex III assembly (PubMed:28673544) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC, IF

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50 IF--1:10~50

Synonyms: Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, RISP, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Complex III subunit IX, Ubiquinol-cytochrome c reductase 8 kDa protein, UQCRFS1